Basic Information                  
Event RT73 (GT73)
OECD UI MON-ØØØ73-7
Trade Name Westar Roundup Ready Canola
Species Brassica napus (Argentine Canola)
Introduced Trait Herbicide Tolerant
Transformation Method Agrobacterium tumefaciens-mediated transformation
Transformation Vector PV-BNGT04
Developer Monsanto Company
Introduced Elements
All Known Sequence:
(1)
ref:    |    AX685146    |
Plant DNA plasmid flanking FMV35S
+ View Complete Sequence & Detailed Infomation
1TTGGTGAGAA GCAGAGAAGA GACACTTAAT ATCCATTAGA TAAAAGGGTG
51TTTCACAAGA GAAATTTGAA AAACTGAACC GAGTAAGCGA AAGAGTGAAG
101AGAGTAATGT AGATTTGCAG AAGTAGAAAG GTGTTTAGGA GCTTAGAAGC
151TTTGATACCA TGAAAGTAAA TGAAGAACAC TGAAGAACAA GAGAAAGAGT
201GATAGATATC TGAGAAATAG AGAGATGTAT TTCAAAACTA AGAGAGAGCG
251TAAATAAGGA TTGATTTCCT TAATTCATAA AATCTGAATA CACTCTATAT
301TTAGACCCCT TAACTATTAA TATACGGGGA TTCGGGGTTG GATCCGGGGC
351GGGTTGTTAC ATAAAAAGTC GAACACTGAT AGTTTAAACT GAAGGCGGGA
401AACGACAATC TGATCCCCAT CAAGCTTGAG CTCAGGATTT AGCAGCATTC
451CAGATTGGGT TCAATCAACA AGGTACGAGC CATATCACTT TATTCAAATT
(2)
ref:    |    AX685147    ||    AJ878609    |
Plasmid Plant DNA
+ View Complete Sequence & Detailed Infomation
1TGAATGTAGA CACGTCGAAA TAAAGATTTC CGAATTAGAA TAATTTGTTT
51ATTGCTTTCG CCTATAAATA CGACGGATCG TAATTTGTCG TTTTATCAAA
101ATGTACTTTC ATTTTATAAT AACGCTGCGG ACATCTACAT TTTTGAATTG
151AAAAAAAATT GGTAATTACT CTTTCTTTTT CTCCATATTG ACCATCATAC
201TCATTGCTGA TCCATGTAGA TTTCCCGGAC ATGAAGATCA TCCTCCTTCC
251TTTCCTTGCC TTTCCTTCCT TTTCTTGCCT TCGTATAAGC TTGTGTCAAT
301TGTTGACAGA GAATCTTGCT GAAGAATTAC TCAGAAACAG AGTACTTCAA
351GGTATTGAAC ATTCCACATG TGGAAATCGA CGGCTAGAAG TAAAAAAAAA
401AGGTAATATT ATTGTGCATA TATATAAAAA CATAACCCCA ACGACCTTAC
451TTGAAGCACA CGACACTGTA ATTC
Target Gene
cp4 epsps cassette
Promotor
CMoVb
figwort mosaic virus (FMV) 35S;chloroplast transit peptide from A. thaliana EPSPS gene (CTP2)
Coding Region
Coding 5-enolpyruvylshikimate-3-phosphate synthase
From Agrobacterium tumefaciens
Terminator
E9 3'
P. sativum (pea) ribulose-1,5-bisphosphate carboxylase small subunit E9 gene
gox cassette
Promotor
CMoVb
figwort mosaic virus (FMV) 35S chloroplast transit peptide from A. thaliana
Coding Region
Coding glyphosate oxidoreductase
From Ochrobactrum anthropi
Terminator
E9 3'
P. sativum (pea) ribulose-1,5-bisphosphate carboxylase small subunit non-translated region
    Molecular Characterization

RT73: 1 insert (1 copy of T-DNA). According to FSANZ, RT73 contains cp4 epsps (1 copy, intact), gox247 gene (1 copy, intact).

Nucleic Acid - Based Method
For Exogenous Gene:
StrategyMethod TypePrimer NameAmpliconProbe TypeValidation InfoStandard
ScreeningQualitativeCAN2 F / CAN2 R385bp
p-FMV04-5 / p-FMV04-3150bp
Gene-SpecificQualitative4EPSPS F / 4EPSPS R274bp
Construct-SpecificQuantitativeGT73-TmF / GT73-TmRUnknownTaqMan
Event-SpecificQualitativeGT73junc-F1 / GT73junc-R1512bp
GT1F / GT1R522bp
GT73-F / GT73-R204bpNational
QuantitativeGT73-a / GT73-b106bpTaqMan
RT73 primer 1 / RT73 primer 2108bpTaqManYes
ChipGT73-AF / GT73-AR118bp
*View All Possible Screen Method
*View All Possible Gene-Specific Method
For Endogenous Reference Gene:
Acc. No: X77576 Copy No (haploid): 2-3
Acc. No: AF127919 Copy No (haploid): 1
Acc. No: X59294 Copy No (haploid):
Acc. No: Copy No (haploid):
phosphoenolpyruvate carboxylase Acc. No: GQ227671 Copy No (haploid):
Protein - Based Method
Certified Reference Materials
Cat. No. Name Average SD
AOCS 0304-B RT73 Roundup Ready™ % %
Fluka 55231 Rapeseed GMO Standard Set % %
*View All CRMs (The CRMs are provided by IRMM, AOCS, and Sigma)
Reference Molecules:
View other Information on Agbios...