Basic Information                  
Event RF3
OECD UI ACS-BNØØ3-6
Trade Name InVigor Canola
Species Brassica napus (Argentine Canola)
Introduced Trait Herbicide Tolerant, Fertility Restoration
Transformation Method
Transformation Vector pTHW118
Developer Bayer CropScience (Aventis CropScience(AgrEvo))
Introduced Elements
All Known Sequence:
    Molecular Characterization

RF3: 1 insertion (2 copy of T-DNA). One copy contains bar and barstar cassettes, the other is an incomplete one (barstar, T-nos and bar gene, without the translation initiation codon).

Nucleic Acid - Based Method
For Exogenous Gene:
StrategyMethod TypePrimer NameAmpliconProbe TypeValidation InfoStandard
Event-SpecificQualitativeRF3-F / RF3-R284bpNational
QuantitativeRf3-RB3 / Rf3-RB8UnknownTaqMan
KVM084 / DPA165UnknownTaqManYes
*View All Possible Screen Method
*View All Possible Gene-Specific Method
For Endogenous Reference Gene:
Acc. No: X77576 Copy No (haploid): 2-3
Acc. No: AF127919 Copy No (haploid): 1
Acc. No: X59294 Copy No (haploid):
Acc. No: Copy No (haploid):
phosphoenolpyruvate carboxylase Acc. No: GQ227671 Copy No (haploid):
Protein - Based Method
Certified Reference Materials
Cat. No. Name Average SD
AOCS 0306-G Rf3 canola % %
*View All CRMs (The CRMs are provided by IRMM, AOCS, and Sigma)
Reference Molecules:
View other Information on Agbios...