Basic Information                  
Event MON89034
OECD UI MON-89Ø34-3
Trade Name
Species Zea mays (Maize)
Introduced Trait Insect Resistant
Transformation Method Agrobacterium tumefaciens-mediated plant transformation
Transformation Vector
Developer Monsanto Company
Introduced Elements
All Known Sequence:
(1)
ref:    
Plant P-35S L-Ta.lhcbl Os.Act CrylA.105 T-Hsp P-FMV Hsp70 leader CTP2 Cry2Ab T-nos Plant
+ View Complete Sequence & Detailed Infomation
1AATATTTAAA AATGGAAGTA ATACTATATT AAAATGATTC ATGTGGAACT
51CCTGCGCTTC TTTTTGAAGT TTCAAAAGGG AGCTTTCAGG GTCGCTTAGA
101GTTTGTTTGG TTGGAAATAC AAGCGAAAAG AGAGCTAATG AGGGGGACAT
151CCATATTTTC TATGGTGTTT GAATAAGAGT CACGCGGGAA TAAGATGAAC
201ACCGAAACAA TTTTTTTGTA GCTACGTGGT TCCAAAAAAT CGAGTAGACG
251GTGTCGCTTC CACCTCATAC TACTTCAACC TCAAACCACA CATCCTTACG
301CGCCCGCCGG TGTCTCCGTC GTCTCAAGTT CTCAACATAC CTACACATGT
351AACCACTACC GGTCTTTGTC ATGTTTCGAA AGAAGATTAC AGGTCCTCTA
401GAAGAGAGGA CGCGGGGTGG CGAGAAAGCT GGGGAAGAAA AAGGCTAGTA
451CATATGATTG GTCTGTGAAC CTGTGAGGTG GGTAGGTAGG TAGGTGGAGA
Target Gene
chs cassette
Promotor
Coding Region
Coding chalcone synthase A
From
Terminator
cry1A.105 cassette
Promotor
P-35S
CaMV 35S 5' untranslated leader from wheat chlorophyll a/b-binding protein, rice actin gene intron
Coding Region
Coding chimeric cry1 delta-endotoxin
From Bacillus thuringiensis
Terminator
T-hsp17.3
3' untranslated region of wheat heat shock protein 17.3
cry2Ab cassette
Promotor
FMV-35S - promoter from Figwort Mosaic Virus Hsp70 intron from maize heat shock protein gene. Transit peptide from maize RBC-small subunit.
Coding Region
Coding cry2Ab delta-endotoxin
From Bacillus thuringiensis
Terminator
nos 3'
nos 3' non-translated terminator from A. tumefaciens
    Molecular Characterization

Nucleic Acid - Based Method
For Exogenous Gene:
StrategyMethod TypePrimer NameAmpliconProbe TypeValidation InfoStandard
Event-SpecificQualitative89034-F / 89034-R207bpNational
M89034-CF / M89034-CR120bp
QuantitativeMON 89034 primer 1 / MON 89034 primer 277bpTaqMan
*View All Possible Screen Method
*View All Possible Gene-Specific Method
For Endogenous Reference Gene:
Acc. No: X07535 Copy No (haploid): 2
maize starch synthase IIb Acc. No: AF019297 Copy No (haploid): 1
high mobility group Acc. No: AJ131373, AF127919 Copy No (haploid): 1
invertase Acc. No: U16123 Copy No (haploid): 1
alcohol dehydrogenase Acc. No: X04050 Copy No (haploid): 1
Phospholipid transfer protein gene Acc. No: Copy No (haploid):
ppi Phosphofructokinase Acc. No: Copy No (haploid):
Protein - Based Method
Certified Reference Materials
Cat. No. Name Average SD
AOCS 0906-A Non-Modified Powder 10g 0g
AOCS 0906-E Powder 10g 0g
*View All CRMs (The CRMs are provided by IRMM, AOCS, and Sigma)
Reference Molecules:
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