Basic Information                  
Event NK603
OECD UI MON-ØØ6Ø3-6
Trade Name Roundup Ready Maize
Species Zea mays (Maize)
Introduced Trait Herbicide Tolerant
Transformation Method Microparticle bombardment
Transformation Vector PV-ZMGT32
Developer Monsanto Company
Introduced Elements
All Known Sequence:
(1)
ref:    |    CS176091    ||    AX342368    |
Plant DNA pMON25496 flanking P-Os.Act1
+ View Complete Sequence & Detailed Infomation
1AATCGATCCA AAATCGCGAC TGAAATGGTG GAAGAAAGAG AGAACAGAGA
51GCCTCACGTT TCCAGGGTGA AGTATCAGAG GATTTACCGC CCATGCCTTT
101TATGGAGACA AGAAGGGGAG GAGGTAAACA GATCAGCATC AGCGCTCGAA
151AGTTTCGTCA AAGGATGCGG AACTGTTTCC AGCCGCCGTC GCCATTCGGC
201CAGACTCCTC CTCTCTCGGC ATGAGCCGAT CTTTTCTCTG GCATTTCCAA
251CCCTAGAGAC GTGCGTCCCT GGTGGGCTGC TCGGCCAGCA AGCCTTGTAG
301CGGCCCACGC GTGGTACCAA GCTTGATATC CCTAGGGCGG CCGCGTTAAC
351AAGCTTACTC GAGGTCATTC ATATGCTTGA GAAGAGAGTC GGGATAGTCC
401AAAATAAAAC AAAGGTAAGA TTACCGGTCA AAAGTGAAAA CATCAGTTAA
451AAGGTGTATA AAGTAAAATA TCGGTAATAA AAGGTGGCCC AAAGTGAA
(2)
ref:    |    CS176092    |
nos 3 Plasmid Maize rps11 and rpoA Genomic DNA
+ View Complete Sequence & Detailed Infomation
1GACGTTATTT ATGAGATGGG TTTTTATGAT TAGAGTCCCG CAATTATACA
51TTTAATACGC GATAGAAAAC AAAATATAGC GCGCAAACTA GGATAAATTA
101TCGCGCGCGG TGTCATCTAT GTTACTAGAT CGGGGATATC CCCGGGGAAT
151TCGGTACCAA GCTTTTATAA TAGTAGAAAA GAGTAAATTT CACTTTGGGC
201CACCTTTTAT TACCGATATT TTACTTTATA CCACCTTTTA ACTGATGTTT
251TCACTTTTGA CCAGGTAATC TTACCTTTGT TTTATTTTGG ACTATCCCGA
301CTCTCTTCTC AAGCATATGA ATGACCTCGA GTAAGCTTGT TAACGCGGCC
351GCCCTAGGGA TATCAAGCTT GGTACCACGC GACACACTTC CACTCTAGTG
401TTTGAGTGGA TCCTGTTATC TCTTCTCGAA CCATAACAGA CTAGTATTAT
451TTGATCATTG AATCGTTTAT TTCTCTTGAA AGCGGTTTCA TTTTTTTTTA
Target Gene
cp4 epsps cassette
Promotor
P-ract1/tact1 intron
P-ract1/ract1 intron containing rice actin 1 promoter, transcription start site;chloroplast transit peptide from A. thaliana EPSPS gene (CTP2)
Coding Region
Coding 5-enolpyruvylshikimate-3-phosphate synthase
From Agrobacterium tumefaciens
Terminator
NOS 3'
A. tumefaciens nopaline synthase (nos) 3'-polyadenylation signal
cp4 epsps cassette
Promotor
P-e35S
enhanced CaMV 35S, maize HSP70 intron;chloroplast transit peptide from A. thaliana EPSPS gene (CTP2)
Coding Region
Coding 5-enolpyruvylshikimate-3-phosphate synthase
From Agrobacterium tumefaciens
Terminator
NOS 3'
A. tumefaciens nopaline synthase (nos) 3'-polyadenylation signal
    Molecular Characterization

NK603: 1 insertion. Contains cp4 epsps (2 copies, intact), enhancer of rice actin promoter (217 bp). And no plasmid backbone sequences are found.

Nucleic Acid - Based Method
For Exogenous Gene:
StrategyMethod TypePrimer NameAmpliconProbe TypeValidation InfoStandard
ScreeningQualitativetNOS 2-5 / tNOS 2-3151bp
Quantitative180-F / 180-R84bpTaqManYesNational
Gene-SpecificQuantitativeAdh primer F / Adh primer R70bpTaqMan
Construct-SpecificQualitativeMon810 1-5 / NK603 1-3444bp
NK603 01-5 / NK603 01-3143bpYesNational
F / R108bp
Event-SpecificQualitativeNK603-1F / NK603-2R108bp
NK603-AF / NK603-AR120bp
NK603L11-5' / NK603L11-3'221bp
QuantitativeNk603mzpF / Nk603mzpR113bpTaqMan
88_17-L / 88_17-RUnknownTaqMan
NK603 primer F / NK603 primer R108bpTaqManYes
ChipNK603-AF / NK603-AR120bp
*View All Possible Screen Method
*View All Possible Gene-Specific Method
For Endogenous Reference Gene:
Acc. No: X07535 Copy No (haploid): 2
maize starch synthase IIb Acc. No: AF019297 Copy No (haploid): 1
high mobility group Acc. No: AJ131373, AF127919 Copy No (haploid): 1
invertase Acc. No: U16123 Copy No (haploid): 1
alcohol dehydrogenase Acc. No: X04050 Copy No (haploid): 1
Phospholipid transfer protein gene Acc. No: Copy No (haploid):
ppi Phosphofructokinase Acc. No: Copy No (haploid):
Protein - Based Method
Commercial Kit
Method Type Taget Gene Product Name Producer
Strip Kitcp4 epspsQuickStix (TM) Strips for Roundup Ready® in CornEnviroLogix, Inc.
cp4 epspsTrait RUR NK603 Test KitStrategic Diagnostics Inc
Certified Reference Materials
Cat. No. Name Average SD
ERM- BF415A NK603 MAIZE (blank) <0.4g/kg g/kg
ERM- BF415B NK603 MAIZE (level 1) 1.0g/kg ±0.4g/kg
ERM- BF415C NK603 MAIZE (level 2) 4.9g/kg ±0.5g/kg
ERM- BF415D NK603 MAIZE (level 3) 9.8g/kg ±0.7g/kg
ERM- BF415E NK603 MAIZE (level 4) 19.6g/kg ±0.9g/kg
ERM- BF415F NK603 MAIZE (level 5) 49.1g/kg ±1.3g/kg
Fluka 06943 Maize GMO Standard ERM-BF415 % %
*View All CRMs (The CRMs are provided by IRMM, AOCS, and Sigma)
Reference Molecules: pMD-ZMp3SNTM-9
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