Basic Information                  
Event T14 / T25
OECD UI ACS-ZMØØ2-1 / ACS-ZMØØ3-2
Trade Name Liberty Link Maize
Species Zea mays (Maize)
Introduced Trait Herbicide Tolerant
Transformation Method Direct DNA transfer
Transformation Vector p35S/Ac
Developer Bayer CropScience (Aventis CropScience(AgrEvo))
Introduced Elements
All Known Sequence:
(1)
ref:    |    AY629235    ||    AX195438    ||    AX695990    |
Plant DNA CaMV Fragment pUC18 P-35S G pUC18 pat linker pUC18 C T-35S pUC18
+ View Complete Sequence & Detailed Infomation
1CGCCGACATT TGGCGCGCCA GGTAGGGGCC TGCCGCGTGT TGACGAACAG
51CTTCCCGTCA AGCTCCAGAT GGGCAGTCTC CAGCAACCTC TCCAGCCCGG
101GACGGTGCTC CGTTTCGGGA GTCTTGAGTT CATGTCCCTC GACGGCAGCT
151ACGACATGAT ACTCCTTCCA CCGCCGTGCG ACAGCGACAA TGGCGGAACG
201ACTCAATGAC AAGAAATATC AAAGATACAG TCTCAGAAGA CCAAAGGGCA
251ATTGAGACTT TTCAAGAAAA TCTTCGTCAA CATGGTGGAG CACGACACGC
301TTGTCTACTC CAAAACAAAG GGTAATATCC GGAAACCTCC TCGGATTCCA
351TTGCCCAGCT ATCTGTCACT TTATTGTGAA GATAGTGGAA AAGGAAGGTG
401GCTCCTACAA ATGCCATCAT TGCGATAAAG GAAAGGCCAT CGTTGAAGAT
451GCCTCTGCCG ACAGTGGTCC CAAAGATGGA CCCCCACCCA CGAGGAGCAT
Target Gene
pat cassette
Promotor
P-35S
CaMV 35S
Coding Region
Coding phosphinothricin N-acetyltransferase 
From Streptomyces viridochromogenes
Terminator
T-35S
CaMV 35S poly(A) signal
Selectable Marker
bla cassette
Promotor
bacterial promoter
Coding Region
Coding beta lactamase
From
Terminator
    Molecular Characterization

T14: 3 copies, contains pat (intact), ori-pUC (intact), bla (partial).

T25: Contains one copy of p35S/Ac, bla (partial), ori-pUC (intact).

Nucleic Acid - Based Method
For Exogenous Gene:
StrategyMethod TypePrimer NameAmpliconProbe TypeValidation InfoStandard
ScreeningQualitativesF / sR79bp
CM01 / CM02220bp
T35S 1-5 / T35S 4-384bp
p35S 1-5 / p35S 1-3101bp
Quantitative35S-promoter.for / 35S-promoter.rev68bpTaqMan
P35S 1-5 / P35S1-3101bpTaqMan
ChipT-35S_29-L / T-35S_29-R134bp
Gene-SpecificQualitativepat1-5 / pat1-3161bp
2F / 2R262bp
QuantitativeKVM-5 / KVM-668bpTaqMan
KVM182 / KVM183136bpTaqMan
Construct-SpecificQualitativeT25 2-5 / T25 2-3311bp
CM01 / PA01400bp
P35S 1-5 / T35S 1-3800bp
T25 1-5 / T35S 4-3200bp
T25-F7 / T25-R3209bpYesISO&National
CM03 / PA01231bpYesNational
T25 1-5 / T25 1-3149bp
QuantitativeT25 1-5 / T25 1-3149bpTaqManYesNational
T25-F / T25-R83bpTaqMan
Event-SpecificQualitativeT25-1F / T25-2R255bp
T25-AF / T25-AR141bp
Quantitative89_12-L / 89_12-R106bpTaqMan
PM1 / revPM1155bpTaqMan
MLD143 / MDB551UnknownTaqManYes
T25-fo / T25-re152bpTaqMan
Chip89_100-L / 89_100-R149bp
T25-AF / T25-AR141bp
*View All Possible Screen Method
*View All Possible Gene-Specific Method
For Endogenous Reference Gene:
Acc. No: X07535 Copy No (haploid): 2
maize starch synthase IIb Acc. No: AF019297 Copy No (haploid): 1
high mobility group Acc. No: AJ131373, AF127919 Copy No (haploid): 1
invertase Acc. No: U16123 Copy No (haploid): 1
alcohol dehydrogenase Acc. No: X04050 Copy No (haploid): 1
Phospholipid transfer protein gene Acc. No: Copy No (haploid):
ppi Phosphofructokinase Acc. No: Copy No (haploid):
Protein - Based Method
Certified Reference Materials
Cat. No. Name Average SD
AOCS 0306-H LibertyLink (T25) corn % %
*View All CRMs (The CRMs are provided by IRMM, AOCS, and Sigma)
Reference Molecules: pMul5pMD-ZM
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