Basic Infomation
Event NK603
OECD UI MON-ØØ6Ø3-6
Trade Name Roundup Ready Maize
Species Zea mays (Maize)
Introduced Trait Herbicide Tolerant
Transformation Method
Transformation Vector PV-ZMGT32
Developer Monsanto Company
Inserted Elements
Target Gene
cp4 epsps cassette
Promotor
P-ract1/tact1 intron
P-ract1/ract1 intron containing rice actin 1 promoter, transcription start site;chloroplast transit peptide from A. thaliana EPSPS gene (CTP2)
Coding Region
Coding 5-enolpyruvylshikimate-3-phosphate synthase
From Agrobacterium tumefaciens
Terminator
NOS 3'
A. tumefaciens nopaline synthase (nos) 3'-polyadenylation signal
cp4 epsps cassette
Promotor
P-e35S
enhanced CaMV 35S, maize HSP70 intron;chloroplast transit peptide from A. thaliana EPSPS gene (CTP2)
Coding Region
Coding 5-enolpyruvylshikimate-3-phosphate synthase
From Agrobacterium tumefaciens
Terminator
NOS 3'
A. tumefaciens nopaline synthase (nos) 3'-polyadenylation signal
All Possible Screening Method
cp4 epspsqualitativesttmf3a / sttmr2a145bp
4EPSPS F / 4EPSPS R274bp
epsps 1-5 / epsps 3-3118bp
epsps 1-5′ / epsps 3-3′118bp
m CP4ES-F / m CP4ES-R333bpNational
Eps-F / Eps-R1199bp
CP4EP-KF1 / CP4EP-KR1202bp
CP4-EPSPS-F / CP4-EPSPS-R320bpNational
Cp4-epsps-F1 / Cp4-epsps-R498bpNational
Cp4-epspsF / Cp4-epspsR513bp
CP4-EPSPS-1F / CP4-EPSPS-2R204bpNational
quantitativecp4 epsps_1-L / cp4 epsps_1-R76bpTaqMan
cp4 epsps_2-L / cp4 epsps_2-R121bpTaqMan
RRS-F / RRS-R84bpTaqMan
Eps-F / Eps-R149bpTaqMan
chipcp4 epsps_12-L / cp4 epsps_12-R145bp
cp4 epsps_27-L / cp4 epsps_27-R182bp