Basic Infomation
Event NK603
OECD UI MON-ØØ6Ø3-6
Trade Name Roundup Ready Maize
Species Zea mays (Maize)
Introduced Trait Herbicide Tolerant
Transformation Method
Transformation Vector PV-ZMGT32
Developer Monsanto Company
Inserted Elements
Target Gene
cp4 epsps cassette
Promotor
P-ract1/tact1 intron
P-ract1/ract1 intron containing rice actin 1 promoter, transcription start site;chloroplast transit peptide from A. thaliana EPSPS gene (CTP2)
Coding Region
Coding 5-enolpyruvylshikimate-3-phosphate synthase
From Agrobacterium tumefaciens
Terminator
NOS 3'
A. tumefaciens nopaline synthase (nos) 3'-polyadenylation signal
cp4 epsps cassette
Promotor
P-e35S
enhanced CaMV 35S, maize HSP70 intron;chloroplast transit peptide from A. thaliana EPSPS gene (CTP2)
Coding Region
Coding 5-enolpyruvylshikimate-3-phosphate synthase
From Agrobacterium tumefaciens
Terminator
NOS 3'
A. tumefaciens nopaline synthase (nos) 3'-polyadenylation signal
All Possible Screening Method
NOS 3qualitativetNOS 2-5 / tNOS 2-3151bp
nosFZMP1 / nosFZMP2125bp
HA-nos118-f / HA-nos118-r118bpYesISO
Nos-1F / Nos-2R180bpYesNational
nos 3_25-L / nos 3_25-R165bp
quantitativeNOS ter 2-5 / NOS ter 2-3151bpTaqManYesNational
P-0217 / P-0218121bpTaqMan
Nos-3F / Nos-4R87bpTaqMan
180-F / 180-R84bpTaqManYesNational
chipHA-Eur118-f / HA-nos118-r118bp
nos 3_30-L / nos 3_30-R138bp